Composite FISH micrograph of SCI-84-829 probe positive cells in full-scale sludge. Target cells in FISH micrograph overlays appear magenta [target probe (Cy3 = red) + EUBmix (Cy5 = blue)] and non-target blue (EUBmix only). Scale bar = 20 microns. - Source:1Aerobic heterotroph
Novel phylotype with no cultured representatives. Cells are observed as small rods distributed throughout the activated sludge flocs 1. MAR-FISH analyses indicate a narrow substrate range, only assimilating pyruvate from those tested. Anoxic carbon uptake indicates possibly ability for fermentation or the use of an unknown residual electron acceptor 1. Inclusion of nitrite in anoxic incubations does not give a noticeable increase in carbon uptake indicating an inability for denitrificaiton 1.
 McIlroy, Awata, Nierychlo, Albertsen, Kindaichi, Nielsen, et al. (2015): Characterization of the In Situ Ecophysiology of Novel Phylotypes in Nutrient Removal Activated Sludge Treatment Plants. PLoS ONE 10 (9): e0136424. doi:10.1371/journal.pone.0136424