Protocols

Please use our optimized protocols for DNA extraction and sample preparation for amplicon sequencing for reproducible results that can be compared across different studies.

DNA extraction from anaerobic digester sludge

DNA extraction from influent wastewater

DNA extraction from activated sludge

MiSeq Sequencing of Amplicons

Barcodes and primers

16S rRNA V1-3 Amplicon Preparation

 

Read more about molecular methods in wastewater treatment in:

Chapter 8: Molecular Methods, Karst, S.M., Albertsen, M., Kirkegaard, R.H., Dueholm, M.S.,Nielsen, P.H.

Experimental methods in wastewater treatment, Loosdrecht, M.C.M. van, Nielsen, P.H., Lopez-Vazquez, C.M., Brdjanovic, D., IWA Publishing (Eds.), 2016. First published. ed. IWA Publishing, London.

You can download the book here.

 

 


The online MiDAS field guide is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

The MiDAS taxonomy is a manual curation of the Silva database and is redistributed for non-commercial use only.

 

Citing MiDAS: McIlroy S.J., Saunders A.M., Albertsen M., Nierychlo M., McIlroy B., Hansen A.A., Karst S.M., Nielsen J.L., Nielsen P.H. (2015) MiDAS: the field guide to the microbes of activated sludge. Database, Vol. 2015

Contact us at mail@midasfieldguide.org